ADCP Reporter Bioassay (THP-1) Technical Manual
Instructions for Use of Product(s)
JA9411, JA9415, JA9511, JA9515
Literature # TM701
The ADCP Reporter Bioassay (THP-1), is a bioluminescent reporter cell-based assay that overcomes the limitations of existing assays. It can be used to measure the potency and stability of antibodies and other biologics with Fc domains that bind and activate FcγRI and/or FcγRIIa. The assay consists of a THP-1 monocyte cell line with endogenous expression of FcγRI and FcγRIIa. This cell line has been genetically engineered to express a NanoLuc® (NL) luciferase reporter driven by FcγR-dependent response elements.
The ADCP Reporter Bioassay Effector Cells (THP-1) are provided in thaw-and-use format as cryopreserved cells that can be thawed, plated and used in an assay without the need for cell culture and propagation. When cocultured with a target cell and relevant antibody, the ADCP Reporter Bioassay Effector Cells (THP-1) bind the Fc domain of the antibody, resulting in promoter-driven luminescence.
This Technical Manual is relevant for Cat.# JA9411 and JA9415; JA9511 and JA9515 (Korea).
Summary of Changes
The following changes were made to the 5/25 revision of this document:
1. Removed an expired patent statement.
2. Revised text about the label in Section 3.
Revised 5/25.