GoTaq® G2 Flexi DNA Polymerase
High-Performance GoTaq® G2 DNA Polymerase with Mg-Free Buffer System
- Full-length Taq DNA polymerase that exhibits 5´→3´ exonuclease activity
- Direct to gel convenience
- Flexibility to optimize Mg concentration in PCR
- Supplied at a concentration of 5u/μl
Catalog Number:
Size
Catalog Number: M7801
Catalog Number: M7805
Catalog Number: M7806
Catalog Number: M7808
GoTaq® G2 Flexi: Freedom to Optimize Mg Concentration
GoTaq® G2 Flexi DNA Polymerase reliably amplifies a wide range of PCR templates and provides high-performing results due to improved manufacturing processes, increased reliability and consistency. GoTaq® G2 Flexi DNA Polymerase is supplied in a proprietary formulation containing 50% glycerol with buffers designed for enhanced amplification. The enzyme is a full-length form of Taq DNA polymerase that exhibits 5´→3´ exonuclease activity.
GoTaq® G2 Flexi DNA Polymerase is supplied with Mg-free 5X Green GoTaq® Flexi Reaction Buffer and 5X Colorless GoTaq® Flexi Reaction Buffer to facilitate Mg2+ titrations for PCR optimization. The 5X Green GoTaq® Reaction Buffer has two dyes (blue and yellow) that separate during electrophoresis to indicate migration progress. The buffer also includes a compound that increases sample density so that samples can be loaded directly onto gels without the need for loading dye. The colorless buffer is used when direct fluorescence or absorbance readings are required without prior purification of the amplified DNA from the PCR.
Performance Guarantee: Promega PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any Promega PCR product, we will send a replacement or refund your account.
Three different targets were amplified with the GoTaq® G2 DNA Polymerase Green (G) and Colorless (C) buffers or competitor products (T, Q, L1, L2 and R) according to manufacturers’ recommended protocols using 3.3ng of input human DNA.
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
MgCl2 |
A351B | 1 × 750μl | 25mM | View Product |
GoTaq® G2 Flexi DNA Polymerase |
M780A | 1 × 100u | ||
|
5X Colorless GoTaq® Flexi Buffer |
M890A | 1 × 1ml | View Product | |
|
5X Green GoTaq® Flexi Buffer |
M891A | 1 × 1ml | View Product |
SDS
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Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Storage Conditions
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
MgCl2 |
A351H | 3 × 1.2ml | 25mM | View Product |
GoTaq® G2 Flexi DNA Polymerase |
M780B | 1 × 500u | ||
|
5X Colorless GoTaq® Flexi Buffer |
M890A | 4 × 1ml | View Product | |
|
5X Green GoTaq® Flexi Buffer |
M891A | 4 × 1ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Storage Conditions
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
MgCl2 |
A351H | 15 × 1.2ml | 25mM | View Product |
GoTaq® G2 Flexi DNA Polymerase |
M780B | 5 × 500u | ||
|
5X Colorless GoTaq® Flexi Buffer |
M890A | 20 × 1ml | View Product | |
|
5X Green GoTaq® Flexi Buffer |
M891A | 20 × 1ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Storage Conditions
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
MgCl2 |
A351H | 60 × 1.2ml | 25mM | View Product |
GoTaq® G2 Flexi DNA Polymerase |
M780B | 20 × 500u | ||
|
5X Colorless GoTaq® Flexi Buffer |
M890A | 80 × 1ml | View Product | |
|
5X Green GoTaq® Flexi Buffer |
M891A | 80 × 1ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.Storage Conditions
Resources
No related resources available
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