Wizard® SV Genomic DNA Purification System
Extracts DNA from Tissues or Cells
- Isolate DNA from cultured cells in 20 minutes
- Choice of spin or vacuum purification protocols
Catalog Number:
Size
Catalog Number: A2360
Catalog Number: A2361
Simple DNA Purification Method for Cells and Tissues
The Wizard® SV Genomic DNA Purification System provides a fast, simple, membrane-based technique for preparing genomic DNA from cultured cells and tissue, including mouse tails. Genomic DNA can be purified from cultured cells in about 20 minutes. Isolation from tissue or mouse tails requires an overnight digestion with Proteinase K (Cat.# V3021). Amplifiable genomic DNA can be isolated from up to 5 × 106 cells, 20mg of tissue or up to 1.2cm of a mouse tail tip without a centrifugation clearing step.
The Wizard® SV Genomic DNA Purification System can be used in either a microcentrifuge (spin) or vacuum protocol. Up to 20 samples can be processed at once in the vacuum format with the Vac-Man® Laboratory Vacuum Manifold (Cat.# A7231) and the Vacuum Adapters (Cat.# A1331).
Sample Types Processed With Wizard® SV Genomic
| Sample Type | Promega | External | Comments |
|---|---|---|---|
|
Peripheral blood mononuclear cells
|
Yes
|
Ezzeldin, H.H. et al. (2005) Clin. Cancer Res. 11, 8699–705.
PubMed ID #16361556. | |
|
Mouse primary granulocytes, macrophages, B lymphocytes and T lymphocytes
|
Yes
|
Morii, E. et al. (2004) Blood 104, 1656–61.
PubMed ID #15172970. |
| Sample Type | Promega | External | Comments |
|---|---|---|---|
|
Mouse liver
|
Yes
|
A protocol is provided in the Wizard® SV Genomic DNA Purification System Technical Bulletin #TB302.
| |
|
Mouse brain
|
Yes
|
A protocol is provided in the Wizard® SV Genomic DNA Purification System Technical Bulletin #TB302.
| |
|
Mouse heart
|
Yes
|
A protocol is provided in the Wizard® SV Genomic DNA Purification System Technical Bulletin #TB302.
| |
|
Mouse spleen
|
Yes
|
Fakhari, F.D. et al. (2006) J. Clin. Invest. 116, 735–42.
PubMed ID #16498502. | |
|
Mouse ear
|
Yes
|
Du, Q. et al. (2006) FASEB J. 20, 1131–41.
PubMed ID #16770012. | |
|
Mouse skin
|
Yes
|
Basal cell carcinoma and noncancerous tissues were frozen at –80°C prior to DNA isolation.
Mancuso, M. et al. (2006) Cancer Res. 66, 6606–14. PubMed ID #16818633. | |
|
Anopheles funestus mosquito carcass
|
Yes
|
Michel, A.P. et al. (2006) Genetics 173, 1389–95.
PubMed ID #16648581. | |
|
Human lung cancer biopsy
|
Yes
|
Sasaki, H. et al. (2005) Clin. Cancer Res. 11, 2924–9.
PubMed ID #15837743. | |
|
Human distant normal colorectal mucosa and tumor tissue
|
Yes
|
Pfeifer, D. et al. (2005) Carcinogenesis 26, 103–7.
PubMed ID #15485994. | |
|
Human frozen thymic epithelial tumor samples
|
Yes
|
Suzuki, E. et al. (2006) Jpn. J. Clin. Oncol. 36, 351–6.
PubMed ID #16762968. | |
|
Human pancreatic islet
|
Yes
|
Del Guerra, S. et al. (2005) Diabetes 54, 727–35.
PubMed ID #15734849. |
| Sample Type | Promega | External | Comments |
|---|---|---|---|
|
Chicken retinospheroid cultures
|
Yes
|
Paraoanu, L.E. et al. (2006) Toxicol. Sci. 89, 314–24.
PubMed ID #16207942. | |
|
HT1080 human fibrosarcoma
|
Yes
|
Cells were transfected with FIV vectors, and genomic DNA was isolated 3 days post-transduction.
Harper, S.Q. et al. (2006) J. Virol. 80, 9371–80. PubMed ID #16973543. | |
|
Monkey primary fibroblasts, virus-infected
|
Yes
|
Cells were infected with ~1 multiplicity of infection (MOI) of pseudotyped HCV and total DNA isolated.
Blanchard, E. et al. (2006) J. Virol. 80, 6964–72. PubMed ID #16809302. |
| Sample Type | Promega | External | Comments |
|---|---|---|---|
|
E. coli K-12 derivative, MC4100
|
Yes
|
Teresa Pellicer, M. et al. (2003) J. Bacteriol. 185, 5815–21.
PubMed ID #13129953. | |
|
Pseudomonas aeruginosa
|
Yes
|
Zhu, H. et al. (2004) Invest. Ophthalmol. Vis. Sci. 45, 1897–903.
PubMed ID #15161855. | |
|
Bacillus subtilis
|
Yes
|
Park, J.H. et al. (2004) J. Bacteriol. 186, 1571–3.
PubMed ID #14973012. | |
|
Eleven Gram-negative bacterial species
|
Yes
|
Modified protocol using the Wizard® SV Genomic Purification System to isolate bacteria as follows: The pelleted bacteria were resuspended in 400µl of enzymatic lysis solution (47mM EDTA, 25mg/ml lysozyme, 20µg/ml lysostaphin) and incubated for 2 hours at 37°C. Next, 19.2 mg/ml proteinase K was added (final concentration 0.4 mg/ml), and the mixture was incubated for 1 hour at 55°C. Finally, the Nuclei Lysis Solution and RNase Solution were added, mixed and incubated for 10 minutes at 80°C.
Zucol, F. et al. (2006) J. Clin. Microbiol. 44, 2750–9. PubMed ID #16891488. | |
|
Eleven Gram-positive bacterial species
|
Yes
|
Modified protocol using the Wizard® SV Genomic Purification System to isolate bacteria as follows: The pelleted bacteria were resuspended in 400µl of enzymatic lysis solution (47mM EDTA, 25mg/ml lysozyme, 20µg/ml lysostaphin) and incubated for 2 hours at 37°C. Next, 19.2mg/ml proteinase K was added (final concentration 0.4mg/ml), and the mixture was incubated for 1 hour at 55°C. Finally, the Nuclei Lysis Solution and RNase Solution were added, mixed and incubated for 10 minutes at 80°C.
Zucol, F. et al. (2006) J. Clin. Microbiol. 44, 2750–9. PubMed ID #16891488. |
| Sample Type | Promega | External | Comments |
|---|---|---|---|
|
Snail cercariae
|
Yes
|
Smith, P. et al. (2004) J. Immunol. 173, 1240–8.
PubMed ID #15240716. |
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Wizard® SV Minicolumns |
A129A | 1 × 50/pk | |
Collection Tubes |
A130A | 1 × 50/pk | |
Column Wash Solution (CWA) |
A1311 | 1 × 185ml | View Product |
|
Nuclei Lysis Solution |
A7941 | 1 × 50ml | View Product |
|
RNase A Solution |
A797A | 1 × 250μl | View Product |
|
Nuclease-Free Water |
P119C | 2 × 25ml | View Product |
|
EDTA, 0.5M (pH 8.0) |
V423B | 1 × 30ml | View Product |
|
Wizard® SV Lysis Buffer |
Z3052 | 1 × 50ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Concentration | Available Separately |
|---|---|---|---|---|
|
Wizard® SV Minicolumns |
A129B | 1 × 250/pk | ||
|
Collection Tubes |
A130B | 1 × 250/pk | ||
Column Wash Solution (CWA) |
A1311 | 2 × 185ml | View Product | |
|
Nuclei Lysis Solution |
A7941 | 1 × 50ml | View Product | |
|
RNase A Solution |
A797C | 2 × 1ml | 4mg/ml | View Product |
|
Nuclease-Free Water |
P1195 | 1 × 150ml | View Product | |
|
EDTA, 0.5M (pH 8.0) |
V423B | 1 × 30ml | View Product | |
|
Wizard® SV Lysis Buffer |
Z3052 | 2 × 50ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
Other Resources
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Frequently Used With
Vac-Man® Jr. Laboratory Vacuum Manifold
For rapid, effective nucleic acid purification. Process two samples at a time.
A7660, A3110
Vac-Man® Laboratory Vacuum Manifold
For fast, effective nucleic acid purification. Process up to 20 samples simultaneously.
A7231
Proteinase K (Lyophilized)
Broad cleavage activity, stability in urea and SDS and ability to digest native proteins.
V3021
QuantiFluor® ONE dsDNA System
"Add-and-read" quantitation of double-stranded DNA. Includes DNA-binding dye, lambda DNA and buffer.
E4871, E4870
Nuclei Lysis Solution
Component of the Wizard®, Wizard® SV and Wizard® SV 96 Genomic DNA Purification Systems available for separate purchase.
A7941, A7943
Wizard® SV Lysis Buffer
Component of the Wizard® SV and SV 96 Genomic DNA Purification Systems used to lyse cells.
Z3052
Column Wash Solution (CWA)
Component of several Wizard® SV DNA Purification Systems available for separate purchase.
A1311, A1318
