Wizard® SV 96 PCR Clean-Up System
96-Well, Vacuum-Based Purification of PCR DNA
- Protocol complete in as little as 20 minutes
- Purifies 100bp to 10kb PCR Products
- 96-well filter plate processing
Catalog Number:
Size
Catalog Number: A9340
Catalog Number: A9341
Catalog Number: A9342
Catalog Number: A9345
The Wizard® SV 96 PCR Clean-Up System is designed for high-throughput purification of 100bp to 10kb PCR products from excess nucleotides, primers and primer dimers. Average recovery is >90% for 100–500bp fragments, and purification is complete in as little as 20 minutes. The product requires use of a vacuum pump and 96-well vacuum manifold (Vac-Man® 96 Vacuum Manifold, Cat.# A2291).
The purified DNA can be used for automated fluorescent sequencing (Phred* 20 scores >600), cloning, labeling, restriction digestion or microarray analysis without further manipulation. The Wizard® SV 96 PCR Clean-Up System uses 96-well filtration without the need to disassemble the manifold. Filtrate waste is delivered directly to a vacuum trap, eliminating the need to dispose of collected waste within the manifold assembly.
Learn more about custom options for this product at: www.promega.com/custom/
*A Phred score is a widely recognized method to measure the quality of DNA sequences. Phred is a base-calling program for DNA sequence traces available from Codoncode Corporation.
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Binding Plate |
A227A | 1 × 1 plate | View Product |
|
Membrane Binding Solution |
A930B | 1 × 20ml | View Product |
|
Nuclease-Free Water |
P119C | 1 × 25ml | View Product |
Collection Plate |
A228 | 1 × 1 plate |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Binding Plate |
A227A | 4 × 1 plate | View Product |
|
Collection Plates (4-pack) |
A9161 | 1 × 1 each | View Product |
|
Membrane Binding Solution |
A9303 | 1 × 100ml | View Product |
|
Nuclease-Free Water |
P1195 | 1 × 150ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Binding Plate |
A227A | 8 × 1 plate | View Product |
|
Collection Plates (4-pack) |
A9161 | 2 × 1 each | View Product |
|
Membrane Binding Solution |
A9303 | 2 × 100ml | View Product |
|
Nuclease-Free Water |
P1195 | 2 × 150ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Wizard® SV 96 Binding Plates |
A2271 | 10 × 10 pack | View Product |
|
Collection Plates (4-pack) |
A9161 | 25 × 1 each | View Product |
|
Membrane Binding Solution |
A9303 | 10 × 100ml | View Product |
|
Nuclease-Free Water |
P1195 | 7 × 150ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
Other Resources
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